Results of Diluting the Dannon Plain Yogurt
June 11th, 2018
Since I could observe the most significant bacterial growth in Dannon plain yogurt in previous lab, I first tried diluting Dannon yogurt plain yogurt before I start to dilute all four brands.
Today's objective was to discover how much bacteria is in each yogurt and count. Yogurt is diluted as 103x and 104x. Then, we extracted 100 μL of culture with two different dilution factors, and plated on 3 plates each.
After incubating all of the plates for more than 48 hours, I counted the number of bacteria in each plate to calculate the colony-forming units (CFU's)
*CFU's in the 1mL of culture = [colonies counted x dilution factor] / total volume plated on dish
Results:
Conclusion:
Since I could observe the most significant bacterial growth in Dannon plain yogurt in previous lab, I first tried diluting Dannon yogurt plain yogurt before I start to dilute all four brands.
Today's objective was to discover how much bacteria is in each yogurt and count. Yogurt is diluted as 103x and 104x. Then, we extracted 100 μL of culture with two different dilution factors, and plated on 3 plates each.
After incubating all of the plates for more than 48 hours, I counted the number of bacteria in each plate to calculate the colony-forming units (CFU's)
*CFU's in the 1mL of culture = [colonies counted x dilution factor] / total volume plated on dish
Results:
 |
| Dannon plain yogurt with dilution factor of 103x and 104x |
Conclusion:
- Dilution factor of 103
- A: 71
- B: 85
- C: 56
- Average number of colony = 70.3
- CFU's = [70.3 x 103] / 0.1mL = 7.03 x 105
- Dilution factor of 104
- A: 8
- B: 6
- C: 5
- Average number of colony = 6.3
- CFU's = [6.3 x 104] / 0.1mL = 6.3 x 105
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