ProGurt Summer II: Fourth Week (7/29 ~ 8/4)
Fourth Week (7/29 ~ 8/4) 7/30/18 MON : Preparation for the Quantification and PCR. 1) Procedure of making buffer solution We needed 900 micro liters because we were doing colonies. 1. Calculate how much solution we need. à For example, if we do 4 colonies, we need 3600 micro liters (900 x 4) 2. Then, calculate the solution 200 micro liters of EDTA 3 micro liters of protein (small one) + 1 micro liters of tween 20 (brown one) = 204 micro liters of solution X N = M (in this case, M should be more than 3600 micro liters) EDTA ...
